WebFeb 27, 2008 · Culture of BJhTERT rho0 and BJhTERT cells Both cell‐types were cultured in a 4 : 1 mixture of Dulbecco’s modified Eagle’s medium and medium 199 supplemented with 10% foetal bovine serum, sodium pyruvate (100 μg/mL), uridine (50 μg/mL) and penicillin/streptomycin (100 IU/mL), in a humidified atmosphere of 5% CO 2 at 37°C. WebDec 11, 2024 · Incumbent Eva Loredo and Jharrett Bryantt advanced to a runoff. They defeated Victor Gonzales in the general election for Houston Community College Board …
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WebCells were stimulated with PDGFBB (Peprotech) for 5 min at 37°C, and the expression of PDGF u0002- receptor and its activation were analyzed by Western blotting using a specific PDGF u0002- receptor antibody (Cell Signaling) and an antibody against phospho-tyrosine (phospho- Y99, sc-7020, Santa Cruz). WebMar 14, 2016 · BJ-hTert, which are human normal foreskin fibroblasts immortalized with hTERT, 48 were purchased from the ATCC (#CRL-4001). 1BR3G human skin fibroblasts … dialysis nursing notes sample
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WebFor RNAseq analysis, SW48, BjhTert, cell lines (1 × 105cells/ well) were seeded in a 6-well plate. Next day, cells were treated with aMan1 (25 μM) for 42 h. Growth protocol: SW48, BjhTert cells were cultured in RPMI (GIBCO) medium supplemented with 10% FCS, 1mM Sodium pyruvate and 1X Glutamax. WebMar 7, 2012 · BjhTERT cells were cultured in IMDM or IMFG supplemented with 10% FBS and PSG. Co-culture assay Tumor cell proliferation on fibroblast monolayers was analyzed in 384-well plates. Fibroblasts were plated in 30–100 μl cell culture medium and cultured for 2–8 days to form confluent monolayers. WebCellosaurus BJ1-hTERT (CVCL_6573) Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE). Doubling time: 36 hours (PubMed= 25984343 ). Transfected with: HGNC; 11730; TERT. Derived from sampling site: … cipro for pouchitis