Cisplatin cck8

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August undefined, 2024

WebCisplatin is a chemotherapy drug that contains the metal platinum. It stops or slows growth of cancer cells and other cells that grow rapidly by damaging their DNA. FDA Approved … WebCCK8 assays Cell viability was measured by cell counting kit-8 (CCK8). The cells were seeded into 96-well plates with 10 μL of CCK8 reagent in every well, and there were 4 × 104 cells in each well, all incubated at 37°C for 1 h. The optical density (OD) values were measured at 450-nm wavelength by Multiskan FC (Thermo Fisher Scientific, Inc.).

lncRNA FOXD2-AS1 confers cisplatin resistance of non-small-cell …

WebAug 8, 2024 · a Before and after treatment with the supernatants of Tregs, cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM) was analyzed by the CCK8 assay. b With different concentrations of Treg supernatants (supernatant frequency of Treg/tumor cell = 1:1, 1:2 and 1:3), cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM ... WebExosome-transmitted circVMP1 facilitates the progression and cisplatin resistance of non-small cell lung cancer by targeting miR-524-5p-METTL3/SOX2 axis Hongya Xie , Jie Yao , Yuxuan Wang & Bin Ni Pages 1257-1271 Received 24 Jan 2024, Accepted 14 Mar 2024, Published online: 25 Apr 2024 Download citation how many kelvins is 100 degrees celsius

The effect of acquired cisplatin resistance on sensitivity to …

WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … WebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was … WebJan 1, 2024 · New therapy targets are urgently needed to reverse DDP resistance. Methods: The protein levels of these molecules were analyzed by Western blotting and immunofluorescent staining. Cell proliferation was detected by CCK8 and MTT assays. Cell migration and invasion were analyzed by wound healing assay and Transwell assays. how many kelvins are in 1 celsius

Exosome-transmitted circVMP1 facilitates the progression and cisplatin …

Cisplatin - NCI - National Cancer Institute

WebCCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of DNA repair-related proteins. Transwell assay was used to detect cell invasion. Results DNA repair was positively associated with the … WebMethods: We use CCK8 assay to show synergistic cytotoxicity of COE and cisplatin. Then, PI single staining and FITC-Annexin V/PI double staining were used to observe apoptotic cells through flow cytometry. The proteins of caspase signaling pathway were examined by Western blotting. howard mees cincinnatiWebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, small cell lung cancer, germ cell tumors, lymphoma, and ovarian cancer. While it is often … howard medical supply

"Web Construction of cisplatin-resistant Siha cell line. (A) Morphology of Siha-N and Siha-DDP. (Magnifications, 200×). (B) Cell viability under the increasing concentration of cisplatin … " - Cisplatin cck8

Cisplatin cck8

Colorectal cancer cell-derived CCL20 recruits regulatory T cells to ...

WebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of... WebMay 27, 2024 · Uncovering the role of p62 in the development of cisplatin resistance in ovarian cancer. ... (IHC) and its proliferation and invasion effects via CCK8 and transwell assays. Additionally, expression of cyclin D1, MMP2, p‐ERK, and EMT‐related proteins was measured by western blotting. We found that SIX1 had significantly higher expression in ...

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Web目的探讨姜黄挥发油(TVO)联合顺铂对人皮肤鳞状细胞癌(鳞癌)A431细胞增殖、凋亡的影响及机制.方法取对数生长期A431细胞,用5、10、20、40、80 mg/L TVO处理,对照组用含有1％二甲基亚砜(DMSO)的DMEM高糖培养基处理,24 h后,CCK8法检测细胞增殖情况.另取部分A431细胞,分为4组,分别用含1％ DMSO的DMEM高糖培养基(对照 ... WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 …

WebSep 30, 2024 · These cell strains were used to determine the effect of HOXB7 on cell viability, and cell apoptosis after exposure to cisplatin. CCK8 assay showed that treatment for 24 hours with cisplatin resulted in decreased IC50 of HOXB7‐knockdown cells compared with control cells (Fig (Fig3a). 3 a). This means that knockdown of HOXB7 … WebMar 27, 2024 · Cisplatin-induced cell killing was analyzed by CCK8 assay. Intracellular ROS levels were detected by fluorescence-based flow cytometry analysis. In vitro overexpression and knockdown of GPX1 expression were performed using GPX1 expression vector and siRNA approaches.

WebFeb 8, 2024 · The rapid development of personalized medicine places high demands on the control of drug dose and cellular drug response to provide patients with better curative effects and low side effects. To solve the problem of low detection accuracies of the cell-counting kit-8 (CCK8) method, a detection method based on surface-enhanced Raman … http://www.rx-8.com/chanpinzhongxin/203370.html

WebFeb 16, 2024 · Generic name: cisplatin [ sis-PLA-tin ] Brand names: Platinol, Platinol-AQ, Platinol (Restricted Access) Dosage forms: intravenous powder for injection (50 mg), …

WebMay 28, 2024 · Non-Small Cell Lung Cancer (NSCLC) patients harboring EGFR activating mutations, such as in-frame deletions in exon 19 (Ex19del) or missense mutation in exon … howard medicineWebSep 18, 2024 · National Center for Biotechnology Information howard medicine costWebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. Transfection of wild-type p53 gene enhanced the ... howard medical technology solutionsWebApr 9, 2024 · Then cells were treated with cisplatin as indicated concentrations. 24 or 48 h later, 10 μL CCK8 reagent (HY-K0301, MedChemExpress, USA) was added into each well for another 2 h. Then the absorbance was measured at 490 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader (Bio Tek). how many kennedys died tragicallyWebFeb 28, 2024 · CCK8 was used in ( a) A549 cells and ( b) H460 cells with different concentrations of cisplatin. ( c) CCK8 was used in A549 and H460 cells transfected with miR-494 or controlled with... how many kennedy childrenWebOct 22, 2024 · Cell viability was analyzed by the Cell Counting Kit-8 (CCK8). The relationship of Nrf2 and CD99 was assessed by dual-luciferase reporter gene assay and chromatin immunoprecipitation (ChIP). Bioinformatics analysis was performed to search for the downstream gene of CD99. how many kelvins are in -19.2ocWebCisplatin-induced acute kidney injury in mice was established by intraperitoneal injection of cisplatin. The levels of urea nitrogen and creatinine in serum were measured， renal pathology change was assayed by HE staining， qRT-PCR and Western blot were used to detect the expression levels of PIM1 mRNA and PIM1 protein. how many kelvins in 60 watt bulb