Dihydroethidium dhe assay
WebDihydroethidium. Catalog No.S6792 Synonyms: DHE, HE, Hydroethidine, PD-MY 003. For research use only. Dihydroethidium (DHE, HE, Hydroethidine, PD-MY 003) is a cell-permeable blue fluorogenic probe used for detecting intracellular superoxide radical anion. CAS No. 104821-25-2.
Dihydroethidium dhe assay
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WebArticle Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of ... WebDihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). [ 1] It has been used to check the in situ generation of ROS …
WebJan 1, 2008 · Dihydroethidium (5-ethyl-5,6-dihydro-6-phenyl-3,8-diaminophenanthridine, hydroethidine, DHE) has been used increasingly as a probe for superoxide in biological systems. ... Since the major DHE-derived product in this assay is 2-EOH, it becomes possible to quantify NADPH oxidase activity with a microplate fluorometric reader … WebDescription. Superoxide indicator dihydroethdium, also called hydroethidine, exhibits blue-fluorescence in cytosol until oxidized, where it intercalates within cell's DNA, staining its nucleus a bright fluorescent red. Excitation Emission: 518/606nm. Cat. No. D23107 packaged 5mM solution stabilized in DMSO.
WebHere, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic … WebWound healing assay was for cell migration. 2ʹ,7ʹ-dichlorodihydrofluorescein diacetate (H 2 DCF-DA) and dihydroethidium (DHE) staining were for ROS generation. Immunofluorescence staining was conducted to assess NF-κB p65. Promoter-reporter gene assay and chromatin immunoprecipitation (ChIP) assay were used to detect promoter …
WebMirella Trinei, ... Marco Giorgio, in Methods in Enzymology, 2013. 6.2 Mitochondrial ROS formation assay. To check whether recombinant p66Shc increases ROS production by isolated mitochondria, we evaluated the oxidation rate of H 2 O 2 − or O 2 · − sensitive dies, respectively, 2′,7′-dichlorodihydrofluorescein diacetate (H 2 DCFDA) and hydroethidin …
WebAbout This Assay Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by … tire shop abbotsfordWebDihydroethidium (DHE) is a widely used sensitive superoxide (O2(*-)) probe. However, DHE oxidation yields at least two fluorescent products, 2-hydroxyethidium (EOH), known … tire shop abbotsford bcWebSep 2, 2024 · Animals and Experimental Models. C57BL/6 mice (male, aged 8 weeks) were obtained from the Animal Core Facility of Nanjing Medical University. They were housed individually in cages under hygienic conditions and placed at a constant room temperature (23 ± 1°C) and humidity (30–40%) and maintained in a 12-h light/dark cycle room with … tire shop achesonWebCell permeant reagent Dihydroethidium (DHE) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS). DHE has been shown to be oxidized by … tire shop abilene txWebApr 7, 2024 · After staining with ROS-specific fluorescent dihydroethidium [DHE; dissolved in dimethyl sulfoxide (DMSO) to 2 mg / mL and diluted with PBS at a ratio ... of bacteria-sourced endotoxin LPS in zebrafish serum was measured using a commercial LPS enzyme-linked immunosorbent assay kit (JL13861; Jonln Biotechnology). To obtain sufficient … tire shop acehWebHere we show that dihydroethidium (DHE) allows the characterization of redox responses upon platelet activation by physiological and pathological stimuli. In particular, the flow … tire shop accessoriesWebArticle Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of ... tire shop advance nc