Earcht-eyfp

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August undefined, 2024

WebExpression of eArchT-eYFP was abundant in MEC-Va terminals located in the PFC, cACC and RSC (Fig. 1B, I, and fig. S2D). These mice were then subjected to contextual fear conditioning (CFC) while we delivered green light bilaterally to the different cortical areas receiving MEC-Va projections WebMay 8, 2014 · Control Data for Optogenetics, eArchT Activation at CA1 Axon Terminal Does Not Affect MEC Unit Firing, Related to Figure 6 (A) An example of MUA in dCA1 with 473 nm light illumination in an anesthetized pOxr1-Cre/eArchT-eYFP mouse. Left, blue bar indicates a period of light illumination (20 Hz for 200 ms).

Ventral CA1 neurons store social memory - PMC

WebSep 30, 2016 · To investigate the potential role of vHPC and dHPC in social memory, we targeted bilateral injections of adeno-associated virus 8 (AAV8)–calcium/calmodulin … WebSep 9, 2024 · (A) Sagittal sections of MEC and CA1 area. MECIII-Cre mice were bilaterally infected with the Cre specific AAVrh8-hSyn1-DIO-eArchT-eYFP virus and silicone linear probes with an optical fiber were implanted in both areas. (B) Examples of light stimulation during quiet awake when animals were in an open arena. Top panel: Light OFF condition. shxybg.com.cn

Engrams and circuits crucial for systems consolidation of a …

WebIf you are using these viruses for the first time, please obtain a Material Transfer Agreement (MTA) by contacting Dr. Karl Deisseroth [email protected] and Charu Ramakrishnan [email protected]. Please submit a copy of your MTA along with your virus order. Web(A to L) Coronal sections of Trpc4-Cre mice injected with AAV9-hsyn:DIO-eArchT- EYFP (A, C), AAVrh8-syn:DIO-EGFP (D to I), and AAV5-hsyn:DIO-GCaMP6f (J to L) into vCA1. WebMar 18, 2024 · The amygdala is a key structure for fear memory (Davis, 1992, Duvarci and Pare, 2014, Ehrlich et al., 2009, Maren and Fanselow, 1996) and is also involved in fear extinction memory ( Amano et al., 2010, Grewe et al., 2024, Herry et al., 2008 ). However, it is unknown whether the amygdala is the storage site of fear extinction memory and, if so ... shxwnt

Addgene: pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

Lateral habenula glutamatergic neurons projecting to the ... - Nature

Web(A) Sagittal sections of MEC regions in the left (L) and right (R) hemispheres of the brain of an MECIII-specific Cre mouse after bilateral injection of … WebTo this end, we made AAV-TetO-eArchT-eYFP-WPRE (TetO::eArchT) , expressing an inhibitory optogenetic actuator activated by green light. Four weeks after injection of TetO::eArchT in Tph2-tTA mice, we tested the effect of photoinhibition of the DRN in the CPP test (Figure 4A–D). To assess aversive properties, we associated green light ... the path beverly hills floridaWebProvide outreach programs, equipment and facilities, and funding for sailing education, training, and competition so that students may learn the life skills and values important in … the path beverly hills

"WebApr 4, 2024 · Specifically, context A-A (trained context) vs. context A-B (neutral context) for natural recall ratios, ChR2-eYFP vs. eYFP for engram cell activation ratios, and eArchT … " - Earcht-eyfp

Earcht-eyfp

MEMORY RESEARCH Engrams and circuits crucial for systems …

WebeArchT-EYFP into vCA1 (Fig. 2, A and B, and fig. S6). We confirmed that the vCA1 neurons speci-fically project to the NAc shell but not to the NAc core, as identified by tyrosine … WebJun 4, 2024 · The role of hyperactivity of LHb neurons after PSL was then investigated by selectively inhibiting LHb Glu using an optogenetic approach (Fig. 2A). pAAV-CaMKIIα …

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WebpLenti-CaMKIIa-eArchT 3.0-EYFP pAAV-CaMKIIa-eArch 3.0-EYFP pAAV-Ef1a-DIO ChETA-EYFP pAAV-Syn-ChrimsonR-tdT Map uploaded by the depositor. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. ... Webinjecting AAV8-CaMKII:eArchT-EYFP into vHPC and then optogenetically inhibiting axonal . 17 . terminals of the vCA1 neurons in the respective target areas while the mice went through SDT . 18 (Fig. 1, L to N, Q to S, and fig. S4). vHPC-NAc projections, but not vHPC-OB or vHPC-BLA, 19 . were essential for social discrimination behavior. 20

WebSep 9, 2016 · Expression of eArchT-EYFP was abundant in vCA1 or dCA1, although it was also observed to a lesser extent in CA3 and the dentate gyrus (DG) (Fig. 1, E and F). … WebSep 9, 2024 · For this purpose, we bilaterally injected Cre-dependent AAVrh8-hSyn1-DIO-eArchT-eYFP into MECIII-specific Cre mice (pOxr1-Cre) and unilaterally implanted a …

WebA Place Where Members are Cherished Like Family. Discover a welcoming atmosphere and warm community to go along with our exquisite dining, premiere racquet facilities, pool … WebNov 18, 2024 · Third, a subset of mice injected with AAV1-DIO-ChR2-EYFP (n = 3) or AAV2/9-DIO-eArchT-EGFP (n = 4) in the OT for optogenetic behavioral experiments was additionally used for anterograde tracing ...

WebMar 21, 2024 · To this end, we made AAV-TetO-eArchT-eYFP-WPRE (TetO::eArchT) , expressing an inhibitory optogenetic actuator activated by green light. Four weeks after injection of TetO::eArchT in Tph2-tTA mice, we tested the effect of photoinhibition of the DRN in the CPP test (Figure 4 A–D). To assess aversive properties, we associated …

WebApr 4, 2024 · Expression of eArchT-eYFP was abundant in MEC-Va terminals located in the PFC, cACC and RSC (Fig. 1B, I, and fig. S2D). These mice were then subjected to contextual fear conditioning (CFC) while we delivered green light bilaterally to the different cortical areas receiving MEC-Va projections during either the conditioning period (Day-1) … the path between us suzanne stabileWebMar 21, 2024 · T o this end, we made AAV-TetO-eArchT-eYFP-WPRE (TetO::eAr chT) [42], expressing an inhibitory. optogenetic actuator activated by green light. Four weeks after injection of TetO::eAr chT in Tph2-tTA. the path blood travels through your bodyWebMay 8, 2014 · Control Data for Optogenetics, eArchT Activation at CA1 Axon Terminal Does Not Affect MEC Unit Firing, Related to Figure 6 (A) An example of MUA in dCA1 with 473 … the pathbreakers from river to oceanWebJul 21, 2024 · d Expression of eArchT-EYFP in the DRN and median raphe nucleus. Optic fiber was placed into the DRN (scale bar 500 μm). The timeline of the experiment was exactly the same as in Fig. ... shxw\u0027ōwhámél first nationWebkinase II (CaMKII):eArchT–enhanced yellow fluorescent protein (eYFP) in the deep layers oftheMECinwild-type(WT)micewithbilat-erallyimplantedoptic fibersabovethePFC,cACC, or RSC (Fig. 1, E and J, and fig. S2G). Expres-sion of eArchT-eYFP was abundant in MEC-Va terminals located in each of these regions (Fig. 1, B and I, and fig. S2D). shxwhay villageWebJul 21, 2024 · d Expression of eArchT-EYFP in the DRN and median raphe nucleus. Optic fiber was placed into the DRN (scale bar 500 μm). The timeline of the experiment was … shxwhl 126.comWebEYFP (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site PacI (not destroyed) 3′ cloning site BsrGI (not destroyed) 5′ sequencing … shxyj.ajcass.org