WebJun 11, 2024 · Fluorescence in situ hybridization (FISH) and immunofluorescence. Rats were perfused through the ascending aorta with 4% paraformaldehyde under anesthesia. The PrL tissues were cut into 25 µm-thick transverse sections after 30% DEPC–sucrose dehydration at 4 ℃ and hybridized at 42 ℃ for 16 h with the 5ʹ-TYE665-label Acp5 probe … WebSep 9, 2024 · RNA fluorescence in situ hybridization (FISH) and antibody staining/immunofluorescence (IF) are widely used to detect distributions of mRNAs and proteins. We would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us.
Combined whole-mount fluorescence in situ hybridization and ... - PubMed
WebHere's a tutorial on how to multiplex a Stellaris RNA FISH assay with an immunofluorescence assay. The method presented in this video is a way to simultaneously perform IF with FISH. An alternative option is to perform IF first and then your Stellaris Assay. Both protocols can be found at our Stellaris Protocols Page. Visit … optimal temperature for brewing tea
Ferroptosis of brain microvascular endothelial cells contributes to ...
WebHere, we describe an experimental procedure for quick sequential immunofluorescence and RNA FISH (immuno-FISH), which enables the simultaneous detection of proteins, chromatin modifications, and RNAs on the inactive X-chromosome (Xi) using female mouse embryonic fibroblast (MEF) and tail-tip 3T3 cell lines. WebHere, we describe an experimental procedure for quick sequential immunofluorescence and RNA FISH (immuno-FISH), which enables the simultaneous detection of proteins, chromatin modifications, and RNAs on the inactive X-chromosome (Xi) using female mouse embryonic fibroblast (MEF) and tail-tip 3T3 cell lines. WebImmunofluorescence and fluorescence in situ hybridization (FISH) are widely used cytogenetic techniques for visualization of protein and RNA/DNA molecules. Here, … portland oregon 10 day