Fluxor herg assay h9c2
WebThe FluxOR™ Potassium Ion Channel Assay is a solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR™ assay takes … WebAfter the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470 ± 20 nm, emission 540 ± 30 nm) followed by a thallium stimulus buffer addition (10 μL/well) and ...
Fluxor herg assay h9c2
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Webs of hERG inhibitors determined in separate tests. N=4. FLIPR Potassium Kit-4 -2 0 2 0 1 3 Dofetilide Terfenadine Pimozide Log[inhibitor] mM) FluxOr Kit-4 -2 0 2 0.0 0.2 0.4 0.6 0.8 1.0 Dofetilide Terfenadine Pimozide Log[inhibitor] mM Compound) IC 50 (nM) FLIPR Potassium Assay 50) FluxOR Assay Dofetalide 39 53 Pimozide 135 195 Terfenadine … WebDec 15, 2010 · In the present study, we describe a high-capacity, 384- and 1,536-well plate, functional thallium flux assay for the hERG channel that fulfills these criteria. This assay was optimized and validated using different structural classes of hERG inhibitors.
Web• The non-homogeneous potassium ion channel assay was run using FluxOR Assay Kit (Life Technologies, Carlsbad, CA) following the manufacturer’s protocol. • All plates were run on FLIPR Tetra Systems using the Calcium Assay setup (Ex 485nm, Em 530nm). Data were acquired at 1 - or 2-second intervals for 140 to 200 seconds. Figure 2. K. V WebJul 16, 2024 · H9C2 cells were subcultured under anoxic conditions to mimic myocardial hypoxia and subsequently treated with salidroside. Whole cell patch clamp was …
WebThe FluxOR assays take advantage of the well described permeability of potassium channels to thallium (Tl+) ions and the utilization of a new and improved indicator dye that results in a larger signal window and a significant improvement in … WebMar 17, 2024 · This assay measures hERG channel activity using thallium ions as surrogate ions. Prior to the experiment, hERG-U2OS or hERG-HEK293 stable cell lines expressing …
WebMTT assay was carried out using H9c2 cells to determine a proper dose range for the designed assay. The ECG measurement revealed that intravenous injection of 4-AcO-DET at 2.0 mg/kg and 4-HO-MET at 1.5 mg/kg prolonged the QTc interval in SD rats. In addition, hERG potassium channels in Chinese hamster ovary cells treated test substances were ...
WebHere, we sought to develop an optical high-throughput assay to monitor surface and total hERG protein expression that would be useful to discern mechanisms underlying LQT2 … chinos and timbsWebThe Predictor™ hERG kit from Invitrogen is a homogeneous fluorescent assay that uses a simple add-and-read format. The assay is based on the principle of fluorescence … chinos and timberlandsWebThe FluxOR™ II Green Potassium Ion Channel Assay is the next-generation solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR II Green assay takes … chinos and tennis shoesWebThe hERG assay, an in vitro electrophysiologic assay, has become routine since its development in the 1990s. It is one of the recommended studies in the ICH S7B regulatory guideline. This is a voltage clamp assay where a cultured mammalian cell (usually human embryonic kidney, or HEK cell) is transfected with hERG DNA. chinos and polo to interviewgranny florist conway scWebFluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium … chinos and tweed jacketWebThree K+ Channels, hERG, Kir2.1, and Kv7.2, were differentially expressed in U-2 OS cells using BacMam technology. Following an overnight incubation cells were dye loaded using FluxOR then incubated with compounds or controls. Each of the four compound plates (Tocris) was tested against the three cell lines hERG, Kir2.1, and Kv7.2. granny flat usa