Hieff pcr
Web2× Plus PCR Master Mix (With Dye) is a ready-to-use 2x premixed solution containing Hieff Canace™ Plus high-fidelity DNA polymerase, dNTPs, and an optimized buffer. This PCR … WebTIANGEN’s has developed various one-tube PCR reaction system (PCR MasterMix), each of which is divided into dye-containing and dye-free systems. Dye-containing MasterMix products can be directly electrophoresed after PCR, without loading buffer.
Hieff pcr
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http://img1.bioon.com/reagent/Show_product.asp?id=4951047 Web1 de nov. de 2024 · The reaction volumes for semiquantitative PCR contained 2.0 μL of cDNA sample, 12.5 μL of 2× Hieff PCR Master Mix, 1.0 μL of both gene-specific primers, and 8.5 μL RNAase-free H 2 O. Semiquantitative PCR was performed with the following cycling standard conditions: denaturation at 94°C for 30 s, annealing at 58°C for 30 s, …
WebCertificate of Analysis GoTaq® Green Master Mix Cat.# Size M7122 100 reactions M7123 1,000 reactions Cat.# M7122 and M7123 include GoTaq® Green Master Mix, 2X, and Nuclease-Free Water. Description: GoTaq® Green Master Mix(a) is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction … Web8 de fev. de 2024 · The composition of the reaction mixture contained 10.0 μL of 2 × Hieff™ PCR Master Mix (Yeasen Biotech Co., Ltd.), 1.0 μL of template, 0.5 μM of each primer, and 8 μL of ddH 2 O. Cycle times were as follows: 95°C for 5 min (initial denaturation), 40 cycles of 95°C for 30 s (denaturation), 54°C for 30 s (annealing), and 72°C for 20 s ...
WebHieff™ Double-Block anti-Taq DNA Polymerase Antibody is a monoclonal antibody to inhibit the activity of 5 '- 3' polymerase and 5 '- 3' exonuclease of Taq DNA Polymerase for hot … Web碱基错误掺入是导致 PCR 意外延伸终止的罪魁祸首. Hieff TM PCR Master Mix 在传统 PCR Mix 基础上优化配方，添加*的校正因子和延伸因子，降低碱基错误掺入率，帮助 Taq …
Web1. PCR reaction system (30 μL) was as follows: DNA template 3 μL (100 ng), upstream and downstream primers 1.5 μL (10 μmol/L) and 2× Hieff™ PCR MasterMix (with dye) 15 μL, with the remaining volume filled with water. 2× Hieff™ PCR MasterMix is a premixed system consisting of Hieff™ HotStart Taq DNA Polymerase, PCR buffer, Mg 2+, dNTPs, and …
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