Mitotimer ミトコンドリア
WebJan 31, 2024 · We demonstrate that the fluorescent protein, MitoTimer, is a reliable and robust probe to follow mitochondrial turnover. The screening of 15 000 small molecules led us to two chemically-related benzothiophenes that stimulate basal mitophagy in the beta-cell line, INS1. Enhancing basal mitophagy was associated with improved mitochondrial ... WebMitoTimer: A novel tool for monitoring mitochondrial turnover. Hernandez G, Thornton C, Stotland A, Lui D, Sin J, Ramil J, Magee N, Andres A, Quarato G, Carreira RS ...
Mitotimer ミトコンドリア
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WebMitoTimer is a mutant of the red fluorescent protein DsRed, in which the fluorescence shifts irreversibly from green to red as the protein is oxidized. They targeted the probe to the mitochondrial matrix with a mitochondrial-targeting sequence. MitoTimer can continuously monitor mitochondrial dynamics within cells and is of particular value ... WebDec 14, 2024 · MitoTimer is a fluorescent protein targeted to the mitochondrial matrix. Upon translation, it emits green fluorescence, but after ∼16 h its emission shifts to red. Thus, the red: green ratio of cells expressing MitoTimer serves as an indicator for mitochondrial turnover, with an increase in the red: green ratio demonstrating a reduction in ...
WebMitochondrial dysfunction plays important roles in many diseases, but there is no satisfactory method to assess mitochondrial health in vivo.Here, we engineered a MitoTimer reporter … WebJan 1, 2024 · When CAG-CAT-MitoTimer mice were crossbred adiponectin-rtTA-TRE-Cre mice, the double transgenic offspring showed MitoTimer expression revealing mitochondrial network in white adipose tissue following 2 weeks of feeding with doxycline-chow (Fig. 2 e). Similarly, when crossbred with PepCK-Cre mice, the double transgenic offspring showed …
WebDec 6, 2014 · MitoTimer also provides information about the level of mitochondrial protein import across a population of cells and organelles. High membrane potential is required … WebJan 17, 2024 · A novel MitoTimer reporter gene for mitochondrial content, structure, stress, and damage in vivo. J Biol Chem 2014; 289: 12005–15. Crossref. PubMed. ISI. Google Scholar. 33. Stotland A, Gottlieb RA. alpha-MHC MitoTimer mouse: in vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart.
WebMay 1, 2016 · The MitoTimer transgene combines the red fluorescent protein, dsRed, and a human cytochrome c oxidase subunit VIII (Cox 8) mitochondrial localization sequence to traffic the protein to the mitochondria where the color shifts from green to red over time (approximately 24 hours). Our goal is to use this as a tool to assess the increase or ...
WebMitoTimer reporter gene. Mitochondria are the power plants in mammalian cells for ATP production essential for cellular processes. Mitochondrial dysfunction is associated with most of the prevalent health disorders, including cardiovascular disease, diabetes, cancer, and neurodegeneration. Impaired mitochondrial function often triggers a ... maine radon testing companiesWebOct 21, 2015 · Furthermore, individual mitochondria within cardiomyocytes display a higher red-to-green fluorescence, implying a block in import of newly synthesized MitoTimer that would be caused by the lack of a high membrane potential, indicative of … maine rank choice voting explainedWebPlasmid pTRE-Tight-MitoTimer from Dr. Roberta Gottlieb's lab contains the insert MitoTimer and is published in Autophagy. 2013 Nov 1;9(11):1852-61. doi: … maine rabies information