WebMay 5, 2008 · The precleared chromatin was collected and incubated at 4°C overnight with 5 μg of anti-Osx antibody, anti-acetylated histone H3 antibody (Upstate Biotechnology) or Rabbit IgG (Sigma) as a negative control. The immune complexes were precipitated with 60 μl of protein G-agarose beads at 4°C for 1 h. WebJul 30, 2024 · Osx is an important factor in bone homeostasis in adults and has roles in degradation of cartilage matrix by directly transcriptionally activating matrix metalloproteinase, ... Zer Vue, Shuo-Ting Yen, and Alejandro Elder Ontiveryos for helpful discussions. pBSOsxBP and OSX antibody were kindly provided by Benoit de Crombrugghe.
Fermented Oyster Extract Promotes Osteoblast Differentiation by ...
WebJan 1, 2024 · Osx, a marker for preosteoblasts, is a zinc finger transcription factor critical for osteoblast differentiation [14]. To demonstrate the utility of cryosections for detection of osteoblast-specific markers, we performed IF staining with Osx antibody. WebFeb 15, 2013 · A. Saos-2 cells were treated with CHX (80 µM) for various periods of time and endogenous Osx protein expression was detected by Western blot analysis with an anti-Osx antibody. B. The expression levels of Osx in panel A were determined by densitometry. The levels of Osx protein in CHX-untreated cells (0 h) were set to 100%. C. eight channel preamp
(PDF) Characterization of Osterix Protein Stability and
WebTherefore, these antibodies are consistent in their structure and performance, leading to high experimental... Anti-Iba-1 Polyclonal Antibody, Unconjugated Anti Iba-1 is specifically reactive to microglia and macrophages, and are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which … WebApr 14, 2024 · Objective: The current molecular classification system for gastric cancer covers genomic, molecular, and morphological characteristics. Non-etheless, classification of gastric cancer based upon DNA damage repair is still lacking. Here, we defined DNA damage repair-based subtypes across gastric cancer and identified clinicopathological, … WebFigure Lengend Snippet: A–E, IHC analysis of Osx expression, sections of tibiae (A–D) and calvariae (E) were immunohistochemically stained using a specific antibody against Osx protein. The nuclei of Osx-positive cells (i.e., osteoblasts) were stained brown. eight channel nvr with hard drive