WebProven TaqMan® probes, which incorporate minor groove binder (MGB) technology at the 3' end, deliver superior allelic discrimination. The MGB molecule binds to the minor groove of the DNA helix, improving hybridization-based assays by stabilizing the MGB probe–template complex. This increased binding stability permits the use of probes WebTaqMan MGB probes incorporate an NFQ to absorb (quench) signal from the fluorescent dye label at the other end of the probe. The properties of the NFQ combined with the short …

TaqMan SNP Genotyping Assays - University of Florida

Webprimer, 100 nM TaqMan MGB probe and 5μL of RNA template. The rRT-PCR assays were carried out using a LightCycler® 480 II (Roche, Basel, Switzerland) under the following conditions: 50°C for 10min, 95°C for 10min, and 45 cycles of 15s at 95°C, 30s at 56°C and 15s at 72°C. Amplification data were collected at 56°C (annealing step) WebDesigning TaqMan® MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express® Software Version 2.0 Overview This tutorial details how to design … cut off值的意义

TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for …

WebAffordable TaqMan Assays for All of Your qPCR Needs Hamburger Menu Button. 登录. 没有账户？建立账号 注册. 产品 ... 1700 Inventoried Probe spans exons Matching Ambion siRNAs: Click here to find siRNAs for Gene TSLP. MicroArray Probes: Agilent Affymetrix ArrayStar ABI Illumina - - - ... WebDual-labeled fluorogenic probes are highly-sensitive, sequence-specific, fluorescent probes designed for real-time quantitative PCR. They can be used with most real-time … WebConfidence in your results is vital when people are counting on you. Detect your target 100% of the time with TaqMan® Assays and reagents. The combination of the most advanced assay design pipeline in the industry and the benefits of MGB probes delivers the specificity you need to detect minor variants across highly homologous targets, or to quantify small … cut off值设定